Development of a semi-nested PCR based method for sensitive detection of tumorigenic Agrobacterium in soil
نویسندگان
چکیده
منابع مشابه
the use of a sensitive rt-nested pcr method for detection of hepatitis c virus
objective: hepatitis c virus is the major cause of viral hepatitis and its diagnosis in suspected specimens is of great importance. the risk of transfusion- transmitted virus infection is primarily the result of failure in serological screening tests to detect recently infected donors in the pre-seroconversion window period of infection. therefore, sensitive and accurate diagnosis of hcv prior ...
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Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...
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Crown gall disease caused by the bacterium Agrobacterium tumefaciens can cause significant economic loss in both commercial walnut orchards and in nursery operations in California. This results from the fact that, Paradox, one of the most popular walnut rootstocks in California, is extremely susceptible to A. tumefaciens infection and Crown Gall formation. By combining direct soil-DNA extractio...
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Polymerase chain reaction (PCR) is a rapid and simple technique with high sensitivity and specificity. Inthe recent years, PCR has been used for rapid detection of viral nucleic acids, such as Humancytomegalovirus (HCMV), whereas, PCR optimization is an important task to be done, especially beforeit’s diagnostic application. Annealing temperature, ion concentration (especially...
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Background and Aims: Viroids are smallest known plant pathogens and cause several economically significant diseases. Until recently, viroid detection relied mainly on biological tests and indexing. Today various diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (RT-PCR) are being used for detection and diag...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2005
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2004.02503.x